NGHIÊN CỨU QUÁ TRÌNH PEROXY HOÁ LIPID TRONG MÔ DA BỊ LÃO HOÁ QUANG BẰNG PHƯƠNG PHÁP HÓA MÔ MIỄN DỊCH

Abstract

Objective: Evaluate the impact of light on lipid peroxidation of skin tissue in various conditions by immunohistochemistry method with two markers MDA AND 4-HNE. Materials and methods: We studed 09 skin samples of human in various conditions by experimental methods and using lighting model aimed at causing photoaging with intensity from1200 lux 2500 lux (warelength from 450nm-760nm) for 350 days. Using immunohistochemical staining technique those skin samples and determinating of 2 markers MDA and 4-HNE before and after illumination experiments. Evaluate the level of expression by quantitative stereoscopic method according to the ratio of marker volume/skin tissue volume on slide. Results: The expression level of 2 markers MDA and 4-HNE increased significantly according to the light exposure area from the range 1200 lux (MDA: 5,69%, 4-HNE: 6,13%) to the range 2500 lux (MDA: 10,95%, 4-HNE: 10,59%) compared to the control group that without illumination to them (MDA: 5,44%, 4-HNE: 5,18%) after 350 days. Conclusion: The process of lipid peroxidation in skin tissue occurs faster and more strongly with the impact of light. Both two markers MDA and 4-HNE increased significantly in photoaging skin tissue.