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EVALUATION OF THE QUALITY CHARACTERISTICS OF COLLECTED UMBILICAL CORD-DERIVED MESENCHYMAL STEM CELLS AT BUU DIEN HOSPITAL
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Abstract
Mesenchymal stem cells (MSCs) are a cell source with great potential in regenerative medicine. However, the clinical efficacy of cell therapy is highly dependent on the quality of these cells. This study evaluated the quality of mesenchymal stem cells from umbilical cord tissue samples collected at Buu Dien Hospital. The results of 12 umbilical cord tissue samples showed that the average cell viability was 99,4±0,25 %, and the average cell number was 17.5±8.05 x106 cells. The expression of surface markers CD105+, CD73+, and CD90+ was above 99%, and the average negative marker was 0.37±0.21%. Microbiological testing showed that all 12 samples were negative for bacteria and fungi. Endotoxin and Mycoplasma testing showed that all samples were negative. Karyotyping of mesenchymal stem cells from different passages showed no changes in number and structure. The results of this study have significant implications for stem cell banking and clinical applications of stem cells.
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Keywords
mesenchymal stem cells, stem cell quality, umbilical cord tissue.
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