NGHIÊN CỨU ĐỘT BIẾN XÓA ĐOẠN GEN LMP1-EBV TRÊN BỆNH NHÂN UNG THƯ BIỂU MÔ TẾ BÀO GAN

Abstract

Hepatocellular carcinoma (HCC) is currently the leading cancer in both incidence and mortality in Vietnam. Recent studies have shown that Epstein-Barr virus (EBV) is associated with tumorigenesis and poor prognosis in HCC patients. The main aim of study was conducted: to determine the prevalence of 30bp deletion mutation in the LMP1 gene, that is an important oncogene of EBV, and the correlation between the LMP1 gene and some paraclinical characteristics in HCC patients. Research method: the cross-sectional study performed on 173 tissue samples derived from HCC patients at the K3 Hospital, Tan Trieu and Hanoi Medical University Hospital. Deletion mutation was detected by PCR method. Results: Our study displayed 36/173 (21%) HCC samples positive with the LMP1 gene; and deletion mutation of LMP1 gene accounted for 18/36 (50%). Additionally, the study identified a correlation between HBV infection and the LMP1 deletion mutation (p=0.018), the concentrations of AFP and AST in HCC patients with LMP1 deletion mutation were higher than wild-type LMP1 gene, but not significantly different (p>0.05). This is the first study to describe the distribution of EBV in HCC patients in Vietnam.