EFFECT OF OCIMUM ESSENTIAL OIL ON MOLD ISOLATED FROM VIETNAMESE SWIMMERS
Nội dung chính của bài viết
Tóm tắt
Introduction: Mold contamination poses a significant threat to various sectors, including food safety, infrastructure, and human health. The increasing resistance of fungi to synthetic antifungal agents necessitates the exploration of natural alternatives. Ocimum gratissimum (L.) essential oil has shown promising antifungal activity. Objective: This study investigated the antifungal properties of Ocimum gratissimum (L.) essential oil on various mold strains. Subjects and methods: The antifungal effectiveness of Ocimum gratissimum (L.) essential oil was evaluated against pathogenic mold strains collected from clinical samples, utilizing a broth dilution technique. Results: Ocimum gratissimum essential oil displayed excellent in vitro antifungal efficacy against the tested pathogenic mold strains. A concentration of 0.6 µL/mL of essential oil completely inhibited 100% fungal isolates. Furthermore, a two-fold dilution (0.3 µL/mL) of Ocimum gratissimum oil was still inhibit Phaeohyphomycetes group. Conclusion: In conclusion, O. gratissimum essential oil demonstrates significant potential for development into novel formulations for the treatment of mold-related diseases.
Chi tiết bài viết
Từ khóa
Ocimum gratissimum (L.) essential oil, mold
Tài liệu tham khảo

2. Sil A, Pramanik K, Samantaray P, Firoz M, Yadav VJJEZS. Essential oils: A boon towards eco-friendly management of phytopathogenic fungi. 2020;8(4):1884-91.

3. Mishra AK, Dubey NK. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and environmental microbiology. 1994;60(4):1101-5. Epub 1994/04/01. doi: 10.1128/aem.60.4.1101-1105.1994. PubMed PMID: 8017906; PubMed Central PMCID: PMCPMC201445.


4. Sharma N, Tripathi A. Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological research. 2008;163(3):337-44. Epub 2006/07/28. doi: 10.1016/j.micres.2006.06.009. PubMed PMID: 16870411.


5. Kumar P, Mahato DK, Gupta A, Pandhi S, Mishra S, Barua S, et al. Use of essential oils and phytochemicals against the mycotoxins producing fungi for shelf-life enhancement and food preservation. International Journal of Food Science and Technology. 2022;57(4):2171-84. doi: 10.1111/ijfs.15563 %J International Journal of Food Science and Technology.


6. Shahina Z, Dahms TES. A Comparative Review of Eugenol and Citral Anticandidal Mechanisms: Partners in Crimes Against Fungi. 2024;29(23):5536. PubMed PMID: doi:10.3390/molecules29235536.


7. Khan A, Ahmad A, Akhtar F, Yousuf S, Xess I, Khan LA, et al. Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity. Research in microbiology. 2010;161(10):816-23. Epub 2010/09/28. doi: 10.1016/j.resmic.2010.09.008. PubMed PMID: 20868749.


8. Kaur M, Tandon R, Kalia A, Chander Mahajan BV, Kairam N. ROS-mediated antifungal activity of Ocimum essential oil-loaded nanoemulsions against postharvest fungal pathogens of Kinnow. Food Bioscience. 2024;57:103429. doi: https://doi.org/10.1016/j.fbio.2023.103429.


9. Singh K, Deepa N, Chauhan S, Tandon S, Verma RS, Singh A. Antifungal action of 1,8 cineole, a major component of Eucalyptus globulus essential oil against Alternaria tenuissima via overproduction of reactive oxygen species and downregulation of virulence and ergosterol biosynthetic genes. Industrial Crops and Products. 2024;214:118580. doi: https://doi.org/10.1016/j.indcrop.2024.118580.


10. Adjou E, Zossoungbo M, Totcheme O, Mohamed S. Essential Oil of Ocimum gratissimum L. (Lamiaceae) as Biopreservative of Peanuts in Post-harvest: Application Model and Effects on Quality of Derived Products. International Journal of Biochemistry Research & Review. 2022:22-8. doi: 10.9734/ijbcrr/2022/v31i830343.


11. Antonescu A-I, Miere F, Fritea L, Ganea M, Zdrinca M, Dobjanschi L, et al. Perspectives on the Combined Effects of Ocimum basilicum and Trifolium pratense Extracts in Terms of Phytochemical Profile and Pharmacological Effects. 2021;10(7):1390. PubMed PMID: doi:10.3390/plants10071390.


12. Phelan MC, Lawler G. Cell counting. Current protocols in cytometry. 2001;Appendix 3:Appendix 3A. Epub 2008/09/05. doi: 10.1002/0471142956.cya03as00. PubMed PMID: 18770655.


13. Karaca N, Koç AN. In vitro susceptibility testing of dermatophytes: comparison of disk diffusion and reference broth dilution methods. Diagnostic microbiology and infectious disease. 2004;48(4):259-64. Epub 2004/04/06. doi: 10.1016/j.diagmicrobio.2003.10.012. PubMed PMID: 15062918.


14. Prakash B, Shukla R, Singh P, Mishra PK, Dubey NK, Kharwar RN. Efficacy of chemically characterized Ocimum gratissimum L. essential oil as an antioxidant and a safe plant based antimicrobial against fungal and aflatoxin B1 contamination of spices. Food Research International. 2011;44(1):385-90. doi: https://doi.org/10.1016/j.foodres.2010.10.002


15. Adjou ES, Kouton S, Dahouenon-Ahoussi E, Soumanou MM, Sohounhloue DC. Effect of essential oil from fresh leaves of Ocimum gratissimum L. on mycoflora during storage of peanuts in Benin. Mycotoxin research. 2013;29(1):29-38. Epub 2013/01/22. doi: 10.1007/s12550-012-0150-y. PubMed PMID: 23334722.


16. Adebolu T, Oladimeji SAJAJoB. Antimicrobial activity of leaf extracts of Ocimum gratissimum on selected diarrhoea causing bacteria in southwestern Nigeria. 2005;4(7):682-4.

17. Marchese A, Barbieri R, Coppo E, Orhan IE, Daglia M, Nabavi SF, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. 2017;43(6):668-89.
