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ASSESSMENT OF THE CHORIONIC VILLI CYTOGENETIC ANALYSIS RESULTS USING TRYPSIN/EDTA AND COLLAGENASE AT HANOI OBSTETRICS AND GYNECOLOGY HOSPITAL
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Abstract
The chorionic villi cytogenetic analysis is the test for abnormal chromosome in placental samples contributing to the explanation of abnormal pregnancies, miscarriage, and stillbirth Objective: To review the results of the chorionic villi cytogenetic analysis on placental tissue using the chemicals Trypsin/EDTA and Collagenase at Hanoi Obstetrics and Gynecology Hospital. Subjects and Methods: The study evaluated the results of the karyotype on 19 placental samples from pregnancies between 6 and ≤ 20 weeks of gestation that were terminated at Hanoi Obstetrics and Gynecology Hospital from November 2023 to August 2024. Priority was given to collecting samples from pregnancies with abnormal ultrasound findings or abnormal prenatal screening results. The variables included gestational age, ultrasound results, and test results. Results: The average gestational age in the study was 11 weeks and 3 days. 84.2% of fetuses showed abnormalities on ultrasound, with hydrops fetalis being the most frequently observed abnormality (37%). The test results showed that 74% of pregnancies had chromosomal abnormalities, with 85.7% of these abnormalities involving chromosomal number irregularities. The most common chromosomal abnormality was monosomy, occurring in 42.8% of cases. The study identified 2 cases of chromosomal structural abnormalities and 1 case of a 69,XXY trisomy.
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Keywords
Chorionic villus chromosome infection, fetal malformation, chromosomal abnormalities in chorionic villi
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