APPLICATION OF FLOW CYTOMETRY TECHNOLOGY IN DIAGNOSIS AND CLASSIFICATION OF ACUTE LEUKEMIA AT CAN THO HEMATOLOGY BLOOD TRANSFUSION HOSPITAL
Main Article Content
Abstract
Background: Flow cytometry was use to analyze cellular immunophenotyping marker can accurately determine the lineages and sub-lineages of acute leukemia, minimizing diagnostic errors based on morphology. Objectives: To determine of lineages and sub-lineages of acute leukemia using flow cytometry. Materials and Methods: Cross-sectional descriptive study on 270 patients diagnosed acute leukemia at Can Tho Hematology and Blood Transfusion Hospital. Results: Adult patients represented the highest proportion (80.7%), predominantly diagnosed with acute myeloid leukemia (AML), while children made up the lowest proportion (19.3%), mostly presenting with acute lymphoblastic leukemia (ALL).Acute leukemia was classified into three lineages: AML for 69.3%, ALL for 30.3%, and no cases of mixed-phenotype acute leukemia were identified. Among the ALL cases, B-cell lineage was more common than T-cell lineage (86.7% vs. 13.3%). The most frequent subtype within B-ALL was common B-cell (55.5%), while in T- ALL, common T and early T subtypes were most prevalent (36.4%). In the AML group, the most common subtypes were M2 (38%), M1 (22.5%), and M4/M5 (18.7%). Conclusions: Flow cytometry was applied to analyze immunophenotypic markers in patients with acute leukemia, primarily of myeloid lineage (69.3%), no cases of mixed phenotype acute leukemia were recorded and specific sublineage classification was feasible.
Article Details
Keywords
Immunophenotyping markers, acute leukemia, flow cytometry
References

2. Lý Tuấn Khải, Hồ Xuân Trường, Trương Thị Minh Nguyệt, Nguyễn Văn Tuệ và các cộng sự (2021), “Nghiên cứu đặc điểm kiểu hình miễn dịch ở bệnh nhân lơ xê mi cấp tại Bệnh viện Trung ương Quân đội 108”, Tạp chí y dược lâm sàng 108,16(DB4), tr.181-189.

3. Phan Hoàng Huy (2020), “Nghiên cứu dấu ấn miễn dịch màng tế bào trong bạch cầu cấp bằng phương pháp đếm tế bào dòng chảy tại bệnh viện trung ương Huế”, Luận van chuyên khoa cấp II, chuyên ngành Huyết học, Trường Đại học Huế.

4. Nguyễn Thanh Bình (2019), “Đặc điểm kiểu hình miễn dịch tủy xương của bệnh nhân Lơ xê mi cấp tại Bệnh viện Nhi Trung ương”, Tạp chí nghiên cứu Y học, 123 (7), tr.158-163.

5. A. C. Shysh, et al. (2018), “The incidence of acute myeloid leukemia in Calgary, Alberta, Canada: a retrospective cohort study”, BMC Public Health.

6. Nguyễn Phương Liên (2012), Ứng dụng kỹ thuật tế bào dòng chảy để đánh giá tồn lưu tế bào ác tính trong bệnh bạch cầu cấp, Luận văn Tiến sĩ y học, chuyên ngành Huyết học, Trường Đại học Y Dược Thành phố Hồ Chí Minh.

7. P Pavithra, et al. (2019), “Transition from morphologic diagnosis to immunophenotypic diagnosis of acute leukemia—experience of establishing a new flow cytometry laboratory”, Journal of Hematopathology, 191-199.
