TÁC ĐỘNG CỦA LASER DIODE 940NM LÊN SỰ TĂNG SINH VÀ DI CƯ CỦA NGUYÊN BÀO SỢI TỪ NIÊM MẠC KHẨU CÁI Ở NGƯỜI

Abstract

Objectives: To evaluate the effects of 940nm diode laser on the proliferation and migration of fibroblasts derived from human palatal mucosa at different energy levels. Materials and Methods: Fibroblasts were obtained from the palatal mucosa of individuals with healthy periodontal tissue. Monolayer cells were irradiated with a 940nm diode laser at various exposure times, including the 4J group (0.1 W for 40 seconds), the 8J group (0.1 W for 80 seconds), and the control group (no irradiation). Cell proliferation was assessed using the MTT assay on days 1, 3, 5, 7, and 9. Wound healing assays were conducted to evaluate cell migration at 8, and 24 hours. The Kruskal-Wallis, Mann-Whitney and Post Hoc tests were used for statistical analysis. Results: The 4J group demonstrated significantly greater cell proliferation compared to the control group on days 7 and 9 (p < 0.05). Meanwhile, the 8J group exhibited enhanced proliferation relative to the control group only on day 7 (p < 0.05) The overall trend in cell migration indicated a gradual increase in cell movement into the gap over time. However, no statistically significant differences were observed among the three groups at 8 and 24 hours. Conclusion: Laser irradiation at 4J and 8J positively influenced the proliferation and migration of fibroblasts derived from human palatal mucosa. This study provides additional evidence on the potential effects of laser therapy in the wound healing process of the palatal area following autologous soft tissue graft harvesting.