TỶ LỆ NGUYÊN BỘI Ở CÁC PHÔI CÓ BẤT THƯỜNG VỀ ĐỘNG HỌC PHÂN CHIA ĐƯỢC ĐÁNH GIÁ QUA HỆ THỐNG NUÔI CẤY PHÔI TIME-LAPSE

Abstract

Background: Time-lapse imaging systems for embryo culture and monitoring enable the detection of abnormal cleavage dynamics that cannot be identified through conventional methods. However, the association between these abnormal morphokinetic patterns and the chromosomal status of embryos remains unclear. This study aimed to evaluate the euploidy rate of embryos exhibiting abnormal cleavage dynamics observed using a time-lapse system, combined with preimplantation genetic testing for aneuploidy (PGT-A). Materials and Methods: A prospective study was conducted on 152 patients undergoing IVF with PGT-A indication at Duc Phuc Hospital from January 2023 to December 2023. Embryos were cultured and monitored using a time-lapse system, recording abnormal morphokinetics such as direct cleavage, reverse cleavage, multinucleation, and vacuole. Blastocysts meeting quality criteria were biopsied for PGT-A, and a single euploid embryo was transferred in frozen embryo transfer (FET) cycles. Data were analyzed using SPSS 20.0, with statistical significance set at p<0.05. Results: Out of 583 blastocysts, 50.4% exhibited abnormal cleavage patterns. The euploidy rate within this group was 45.2%. Specifically, multinucleated embryos had a euploidy rate of 38.2%, which was significantly lower compared to aneuploid embryos (p<0.05). Similarly, reverse cleavage observed during the first cell cycle showed a euploidy rate of 33.8%, also significantly lower than the aneuploid rate (p<0.05). Other abnormalities, such as direct cleavage and vacuole presence, did not significantly increase aneuploidy rates. Conclusion: Time-lapse systems provide valuable insights into abnormal cleavage dynamics; however, not all abnormalities are associated with chromosomal abnormalities. Multinucleation and reverse cleavage in the first cell cycle were associated with higher aneuploidy rates, while direct cleavage and vacuole formation did not significantly impact chromosomal status.