HIỆU QUẢ PHỐI HỢP KHÁNG SINH IN VITRO TRÊN VI KHUẨN GRAM ÂM KHÁNG CARBAPENEM

Abstract

Background: According to the World Health Organization, Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae carbapenem - resistance (APK-CR) are the most dangerous bacteria. The new antibiotic development should be prioritized for APK-CR because of their antibiotic resistance seriously. Objective: Evaluate the resistance of APK and the effectiveness of in vitro antibiotic combination of meropenem (ME) - colistin (COL) and meropenem - ciprofloxacin (CIP) to these bacteria strains. Methods: Descriptive and analytical cross sectional study. APK-CR strains were collected at University Medical Center in Ho Chi Minh City from December 2020 to June 2021. Results: There were 151 APK-CR strains (51 A. baumannii, 50 P. aeruginosa and 50 K. pneumoniae). The MICs of ME and CIP on APK-CR strains were both high (accounting for 92-100%); 6% of P. aeruginosa and 10% of K. pneumoniae were MIC resistant to COL. The combination of ME-COL gave high synergistic and additive effectiveness on A. baumannii at the rate of 58.8% and 41.2%, while P. aeruginosa showed the lower rate of 32% and 60%, K. pneumoniae was at the rate of 20% and 60%. The combination of ME-CIP gave high synergistic and additive effectiveness on K. pneumoniae at the rate of 42% and 44%, while A. baumannii showed the lower rate of 33.3% and 45.1%, P. aeruginosa was at the rate of 30% and 60%. Conclusion: APK-CR resists to ME and CIP with a very high rate. The antibiotic combination in vitro of ME-COL and ME-CIP on APK-CR has the synergistic and additive effectiveness.