KHẢO SÁT TÁC DỤNG CẢI THIỆN TRÍ NHỚ CỦA VIÊN NÉN ĐAN SÂM TAM THẤT TRÊN MÔ HÌNH IN VIVO VÀ IN VITRO

Abstract

Backgrounds: Cognitive decline, also known as memory impairment, progressive dementia, can severely impact daily activities. Drugs targeting the inhibition of acetylcholine enzymes are limited in their effectiveness. Therefore, research aims to assess the memory-enhancing effects of DSTT (name of a drug or treatment) tablets in vitro and in vivo. Objective: Investigate the potential for memory improvement of DSTT tablets in in vivo and in vitro. Materials and methods: The DSTT tablets contain 52.5 mg of Radix Salviae mirhizae and 20 mg of Radix Panasis notoginseng extract. In the study, the Ellman's reagent method was used to screen the in vitro AChE inhibitory effects of DSTT tablets. The Morris water maze model was employed to evaluate the memory-enhancing effects of DSTT tablets on scopolamine-induced memory-impaired mice. Results: The DSTT tablets have the ability to inhibit acetylcholinesterase with an IC50 = 28.16 mg/mL. In the Morris water maze model, mice with memory impairment induced by scopolamine were administered DSTT at doses of 1 tablet/kg, 1.5 tablets/kg, and 2 tablets/kg continuously for 23 days. The results showed the potential for restoring long-term memory by increasing the time spent in the platform's ¼ quadrant: 73.91 (p<0.05); 134.38 (p<0.05); 169.88 (p<0.05). Conclusions: DSTT tablets with dose levels of 1 tablet/kg; 1.5 tablets/kg; 2 tablets/kg has the ability to restore proximal memory in patients with memory impairment caused by scopolamine.