ỨNG DỤNG KỸ THUẬT SẮP XẾP DÃY MÔ TRONG NGHIÊN CỨU CÁC DẤU ẤN HÓA MÔ MIỄN DỊCH Ở U LYMPHO TẾ BÀO B LỚN LAN TỎA

Abstract

Objective: Comparing the results of some markers when immunohistochemical staining using the tissue microarray technique with traditional techniques at the Department of Pathology, Hospital K Quan Su. Subject and method: A descriptive, cross-sectional study has been conducted on 30 patients with DLBCL who were surgically treated and pathologically diagnosed at the K hospital. Result: IHC staining using tissue microarray and traditional techniques had similar rates of revealing markers, with very little difference in markers CD20 and MUM-1; in 30 cases of lymphoma. diffuse large B cells, the proportion under the germinal center type is 76.7%. Conclusion: There was no statistically significant difference between the rate of expression of CD20, CD79a, CD10, BCL6 and Ki67 markers between the tissue micorarray technique and the traditional technique (p>0.05). DLBCL – nGCB accounts for as high as 76.7% and DLBCL – GCB accounts for 23.3%. Tissue sequencing techniques can be considered for application in pathology laboratories to perform research while still ensuring the accuracy of results.