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EVALUATION OF THE ACUTE AND SUB-CHRONIC TOXICITY OF DANSHEN - SANQI TABLETS ON EXPERIMENTAL ANIMAL MODELS
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Abstract
Objective: To assess the acute chornic and the sub-chronic toxicity of the DanShen (Salvia miltiorrhiza) and SanQi (Panax notoginseng) tablet (ĐSTT) on Swiss albino mice. Subjects: The coated tablets of Dan-Shen and San-Qi were provided by DOMESCO Medical Import-Export Joint Stock Company. Method: Acute toxicity was assessed by fasting mice (50% male, 50% female) for 12 hours before administering the maximum possible oral dose (up to 0.2ml/10g). General movements, behavioral expressions, cage conditions, eating and drinking habits, defecation and urination, and the number of deceased mice were observed and recorded over 72 hours. The sub-chronic toxicity of DSTT capsules was conducted on white mice, which were divided into 6 groups: Group 1 - BT (physiological control): water; Group 2 - BL (pathological control): water and injected with 1mg/kg scopolamine subcutaneously; Group 3 - DON (positive control): Drinking Donepezil at a dose of 5 mg/kg; Group 4 - DSTT1 (low dose): DanShen - SanQi tablet at a dose of 1 tablet/kg; Group 5 - DSTT2 (medium dose): DanShen - SanQi tablet at a dose of 1.5 tablets/kg; Group 6 - DSTT3 (high dose): DanShen - SanQi tablet at a dose of 2 tablets/kg. Results: Regarding acute toxicity, the maximum dose that can be administered via a blunt-end needle directly into the mouse's stomach is 100 tablets/kg, equivalent to 31,654 mg of the drug powder/kg, with no abnormal signs in behavior or physiology observed in all experimental mice. The results showed that at all doses, the DSTT capsules led to congested liver tissue, slight lymphocyte infiltration, and few neutrophils around the blood vessels in the interstitial tissue. At a high dose (2 tablets/kg), scattered necrosis was observed. However, no histological changes in the kidneys were noted in all 6 groups investigated. Conclusion: In the experiment, the dosages of 2 tablets/kg; 1.5 tablets/kg; 1 tablet/kg are within the safe range for mice. The study on sub-chronic toxicity at various doses showed no changes in the histological structure of the kidneys in white mice during the research period. However, pathological changes in the liver were observed at high doses, requiring further research to assess the impact of the DanShen - SanQi tablet on liver pathology.
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Keywords
Capsule, ĐSTT, liver and kidney histology
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