NGHIÊN CỨU PHÁT TRIỂN KIT MIỄN DỊCH PHÁT HIỆN NHANH PATULIN TRONG THỰC PHẨM QUY MÔ PHÒNG THÍ NGHIỆM

Abstract

Objectives: Determine some technical parameters of Lateral flow immunoassay for rapid detection of Patulin. Methods: Optimal parameters include: concentration of antibody covering the gold nanoparticle, buffer solution applied to the test strip, and stability of the antibody on the conjugate membrane. Results: The optimal antibody concentration is 20 µg/ml; and running buffer is MES (50mM, pH 6.7). The stability of the antibody on the conjugate membrane is 3 months. Conclusions: By optimizing the optimal antibody onto the gold nanoparticle, optimizing the conjugate membrane treatment solution, and optimizing the running buffer, we have developed Lateral flow immunoassay to quickly detect Patulin.