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RESEARCH ON THE CHARACTERISTICS OF BONE MARROW CELLS AND AUTOLOGOUS BONE MARROW STEM CELLS ISOLATED FROM BONE MARROW FLUID OF PATIENTS WITH KNEE OSTEOARTHRITIS
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Abstract
Background: From 11/2011 to 10/2013, forty-six patients with forty-six knee osteoarthritis (KOA) underwent Arthroscopic Subchondral Drilling (microfracture) in combination with Autologous Bone Marrow Stem cells (ABMSCs) injection. Objectives: 1) Investigate some indexes of ABMSCs block of patients with KOA. Subjects and Methods: Forty-six primary KOA underwent ASD in combination with ABMSCs injection from pelvis. Most of knees had grade 3 cartilage lesions. Results: The average number of nucleated cells was 69.03 ± 49.86 G/L, in which the average percentage of CD34 (+) stem cells used 0.71 ± 0.78%. The number of CD34(+) cells obtained in Autologous Bone Marrow blocks average 8,15 ± 0,67 x106. The number of cells that create clusters of CFU-F average 33,34 ± 39,98 x103. Conclusion: tem cells obtained from bone marrow fluid of patients with primary knee osteoarthritis stage 2 and 3 without culture can be used effectively in the treatment of knee osteoarthritis.
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Keywords
Osteoarthritis, bone marrow stem cell, arthroscopy, microfracture
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