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Date Published: 22/07/2024
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DOI: https://doi.org/10.51298/vmj.v540i3.10519
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Vol. 540 No. 3 (2024)
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Các bài báo
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Nguyễn , T. H. H., & Bùi, K. C. (2024). EVALUATION OF TNF-ΑLPHA SECRETION CAPACITY OF CAR-T CELLS IN COMBINATION WITH ANTI-PD-1 ANTIBODIES IN VITRO. Vietnam Medical Journal, 540(3). https://doi.org/10.51298/vmj.v540i3.10519

EVALUATION OF TNF-ΑLPHA SECRETION CAPACITY OF CAR-T CELLS IN COMBINATION WITH ANTI-PD-1 ANTIBODIES IN VITRO

Thị Hiền Hạnh Nguyễn , Khắc Cường Bùi

Main Article Content

Abstract

Objective: To evaluate the TNF-α secretion capacity of CAR-T cells in combination with anti-PD-1 antibodies. Materials and methods: We performed a prospective experimental study on 3 cell lines, including 2 CD19(+) leukemia cell lines (Daudi and Raji) and 1 CD19(-) cell line (K562). All 3 cell lines were co-cultured with 4 groups of PBMC (Peripheral Blood Mononuclear Cell), anti-PD-1 antibody (PD-1 Ab), CAR-T cells only, and CAR-T cells in combination with anti-PD-1 antibodies. The TNF-α secretion capacity at 24 and 48 hours after co-culture was determined using the TNF-α Human ELISA Kit from Thermo Fisher Scientific. Results: At both 24 and 48 hours, TNF-α concentrations in the group of CAR-T cells only and CAR-T cells in combination with anti-PD-1 antibodies when co-cultured with CD19(+) leukemia cell lines (Daudi and Raji) were significantly higher than in the PBMC cells (control group) co-cultured with CD19(+) leukemia cell lines and the CD19(-) cell line. However, there was no difference in TNF-α concentrations between the CAR-T cell only and CAR-T cells in combination with anti-PD-1 antibodies (p > 0.05). Conclusion: TNF-α concentrations increased in culture media of CAR-T cells and CAR-T combined with PD-1 Ab when co-cultured with cancer cells. Especially when cultured with CD19(+) cells, TNF-α concentrations increased higher than when cultured with CD19(-) cells.

Article Details

Keywords

CAR-T, PD-1 Ab, TNF-α

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