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TO INVESTIGATE CHARACTERISTICS OF PATIENT USING NON-INVASIVE PRE-IMPLANTATION GENETIC TEST
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Abstract
Objectives: To investigate characteristics of patient using non-invasive pre-implantation genetic test. Subject and methods: This descriptive cross-sectional study was conducted on 66 volunteer couples with indication for PGT-A và NiPGT-A from 2020 to 2023 at the Military of Institute Clinical Embryology and Histology, Tam Anh Hospital, Nghe An General Hospital; embryos were cultured by using single-drop culture process. Results: The mean age of 66 patients was 35,20 ± 4,12; secondary infertility accounts for 86,4%; Main indications for PGT-A and NIPGT-A mainly include consecutive implantation failure (22,72%) and advanced maternal age (59,09%); the mean level of FSH: 6.66 ± 2.01 (mIU/ml), LH 5.87 ± 2.91 (mIU/ml), E2 40.03 ± 34.35(mIU/ml), P4: 0.21 ± 0.19(mIU/ml), Prolactin 185.2 ± 231.22; the mean number of Antral follicles count was 16.27 ± 9.22; total FSH dose used in controlled ovarian stimulation cycle was 2567.42 ± 452.84IU, the mean duration of FSH administration was 10.12 ± 0.87days; the average number of aspirated oocyte corona complexes was 13.82 ± 7.72 complexes. MII oocyte was 10.72 ± 6.4; the mean 2PN zygote was 8.77 ± 5.53; the mean MII oocyte rate was 79 ± 17,4%; the fertilization rate was 82,81±15,96%; the percentage of day-3 cleavage embryo was 93,75± 14,87%, the blastulation rate was 62,23 ± 22,4%. Conclusions: Main indications for PGT-A and NIPGT-A mainly include consecutive implantation failure (59,1%) and advanced maternal age (22,7 the percentage of day-3 cleavage embryo was 93,75± 14,87%, the blastulation rate was 62,23 ± 22,4%.
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Keywords
single-drop embryo culture, individual embryo culture, in vitro fertilization, niPGT, noninvasive pre-implantation genetic testing.
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