ĐIỀU TRỊ THƯƠNG TỔN DA GIỐNG VẢY NẾN TRÊN MÔ HÌNH CHUỘT BẰNG TIÊM TĨNH MẠCH TẾ BÀO GỐC TỪ MÔ MỠ CÙNG LOÀI

Abstract

Introduction: Mesenchymal stem cells (MSCs) are adult stem cells that can be isolated from various sources, with adipose tissue being a plentiful and easily accessible source from adults. Numerous studies have demonstrated that adipose-derived stem cells (ADSCs) possess immunomodulatory properties, making them effective in treating immune-related disorders. Our study evaluates the therapeutic efficacy of intravenously administered ADSCs in improving psoriasis-like skin lesions in a mouse model. Methods: The study was conducted on five groups of Balb/c mice, with 30 mice in each group: normal mice, IMQ-induced psoriasis model mice (IMQ), psoriasis model mice treated with PBS injection (IMQ+PBS IV), psoriasis model mice treated with topical corticosteroids (IMQ+TCS), and psoriasis model mice treated with intravenous ADSC injection (IMQ+ADSC 2.5 x 10⁶IV). Evaluations included the modified Psoriasis Severity and Activity Index (PASI) for mice, histopathological analysis, and the measurement of key cytokines IL-17A and IL-23 expression levels within the psoriatic lesions on day 6. Results: The ADSC-treated group showed a statistically significant reduction in psoriasis severity compared to IMQ group and the IMQ+PBS IV group. Histopathological features characteristic of psoriasis, epidermal thickness, and expression of IL-17A and IL-23 in the skin were markedly improved in the IMQ+ADSC 2.5x10⁶ IV group. These features were also significantly improved in the IMQ+TCS group; however, mice in this group developed erythema and telangiectasia in the dorsal skin. Conclusion: Intravenous administration of ADSCs can improve psoriasis-like skin lesions in a mouse model without the side effects associated with topical steroid application.