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Date Published: 30/09/2024
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DOI: https://doi.org/10.51298/vmj.v542i3.11220
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Vol. 542 No. 3 (2024)
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Đào, N. B., Tạ, B. T., Nguyễn, C. T., & Võ , T. B. T. (2024). RESULTS OF OF DETECTION OF MYCOBACTERIA TUBERCULOSIS BY 16S RRNA-REAL-TIME EXBP-RT-PCR TEST IN PATIENTS WITH NEWLY DIAGNOSED PULMONARY TUBERCULOSIS AFB SMEAR-NEGATIVE. Vietnam Medical Journal, 542(3). https://doi.org/10.51298/vmj.v542i3.11220

RESULTS OF OF DETECTION OF MYCOBACTERIA TUBERCULOSIS BY 16S RRNA-REAL-TIME EXBP-RT-PCR TEST IN PATIENTS WITH NEWLY DIAGNOSED PULMONARY TUBERCULOSIS AFB SMEAR-NEGATIVE

Ngọc Bằng Đào, Bá Thắng Tạ, Chí Tuấn Nguyễn, Thị Bích Thủy Võ

Main Article Content

Abstract

Objective: To evaluate the results of the the results of detection of Mycobacteria tuberculosis in bronchial lavage fluid by 16S rRNA-real-time ExBP-RT-PCR test in patients with newly diagnosed pulmonary tuberculosis AFB smear-negative. Subjects and methods: A prospective, descriptive, cross-sectional study was conducted on 30 patients with newly diagnosed pulmonary tuberculosis AFB smear-negative and 30 community-acquired pneumonia ones, treated as inpatient at the Respiratory Center, Military Hospital 103, from September 2021 to January 2024. Evaluating the sensitivity and specificity of the 16S rRNA-real-time ExBP-RT-PCR test in detection of Mycobacteria tuberculosis, comparing with the results of the GeneXpert/Mtb-Rif test. Results: Results of 16S rRNA-real-time ExBP-RT-PCR test were positive in 25 patients (83,3%). The sensitivity and specificity of the test follow 83.3% and 100%, respectively. The positive proportion of the 16S rRNA-real-time ExBP-RT-PCR test is higher than GeneXpert/Mtb-Rif but not statistically significant (p > 0.05). Conclusions: Intitial result of 16S rRNA-real-time ExBP-RT-PCR test has high sensitivity, specificity in detection of Mycobacteria tuberculosis in patients with newly diagnosed pulmonary tuberculosis AFB smear-negative.

Article Details

Keywords

Newly diagnosed pulmonary tuberculosis AFB smear-negative; 16S rRNA; PCR.

References

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