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VALUE OF THE 16S RRNA-REAL-TIME EXBP-RT-PCR TEST IN NEWLY DIAGNOSED SPUTUM POSITIVE PULMONARY TUBERCULOSIS
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Abstract
Objective: To evaluate the results of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of newly diagnosed sputum positive pulmonary tuberculosis. Subjects and methods: A prospective, descriptive, cross-sectional study was conducted on 30 newly diagnosed sputum positive pulmonary tuberculosis patients and 30 community-acquired pneumonia patients, treated as inpatient at the Respiratory Center, Military Hospital 103, from September 2021 to January 2024. Evaluating the sensitivity and specificity of the 16S rRNA-real-time ExBP-RT-PCR test in diagnosis of pulmonary tuberculosis, comparing with the results of the GeneXpert/Mtb-Rif test. Results: Results of 16S rRNA-real-time ExBP-RT-PCR test were positive in 30 patients (100%). The both sensitivity and specificity of the test achieve 100%, respectively. The positive proportion of the 16S rRNA-real-time ExBP-RT-PCR test is higher than GeneXpert/Mtb-Rif but not statistically significant (p > 0.05). Conclusions: Intitial result of 16S rRNA-real-time ExBP-RT-PCR test has high sensitivity, specificity in diagnosis of newly diagnosed sputum positive pulmonary tuberculosis.
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Keywords
Newly diagnosed sputum positive pulmonary tuberculosis; 16S rRNA; PCR.
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