ĐÁNH GIÁ CÁC PHƯƠNG PHÁP PHÁT HIỆN STAPHYLOCOCCUS AUREUS KHÁNG METHICILLIN TẠI BỆNH VIỆN ĐA KHOA THÀNH PHỐ CẦN THƠ

Abstract

Objective: Use methods to detect Methicillin-resistant S. aureus at Can Tho City General Hospital to determine the rate of MRSA and evaluate MRSA detection methods. Methods: Cross-sectional descriptive study combined with laboratory on 81 S.aureus strains isolated through routine bacterial culture tests at the Department of Microbiology, Can Tho City General Hospital, Correctly identify S.aureus bacteria from clinical specimens, perform antibiograms and MRSA screening tests using 3 techniques: oxacillin plate, Cefoxitin plate and PCR to find mecA. Results: 81 S. aureus strains isolated in 2023-2024 at Can Tho City General Hospital were quite resistant to Clindamycin (83.0%) and Erythromycin (81.8%). However, it is still highly sensitive to Linezolid (100.0%), Tygecycline (100.0%). In particular, there were 4 strains resistant to Vancomycin (4.9%). The proportion of MRSA strains by the agar diffusion method using Oxacillin and cefoxitin plates was 87.5%, however, the PCR technique identified 65 S.aureus strains carrying the mecA gene (80.2%) lower than that of MRSA strains. Cefoxitin disc technique (87.5%). Comparing the mecA-mediated MRSA identification technique with Cefoxitin disk, the accuracy: 73/81= 90.1%; Specificity: 8/8=100%; Sensitivity: 65/73=89.1%. Conclusion: Using Cefoxitin antibiotic discs to identify mecA-mediated MRSA has accurate results, simple techniques, and is easy to perform in all microbiology laboratories.