ĐÁNH GIÁ SỰ HÌNH THÀNH MÀNG SINH HỌC TRÊN KÍNH ÁP TRÒNG ẢNH HƯỞNG NGUY CƠ BỆNH LÝ VIÊM GIÁC MẠC

Abstract

Objective: Evaluation of biofilm formation on contact lenses in use in Vietnam and its association with keratitis: a study of treatment approaches using eye drops and contact lens solutions. Subjects and methods: Determine the amount of biofilm on contact lenses using the colony counting method after exposing the contact lenses to microorganisms for 24 hours. Treat the contact lenses with biofilm using ophthalmic solutions for 30 minutes, then determine the remaining amount of biofilm using the colony counting method. Results: The lowest microbial density capable of forming biofilm on contact lenses is 10³ CFU/ml. Tobramycin 0.3% eye drops (Traphaco) exhibit excellent biofilm resistance on contact lenses (100%). Sodium Chloride 0.9% eye drops (Pharmedic) show about 30% biofilm resistance against S. aureus, 80% against P. aeruginosa, and 50% against C. albicans. SEED Forest Leaf EX contact lens soaking solution resists 30-40% of S. aureus biofilm, about 70% of P. aeruginosa biofilm, and has poor resistance against C. albicans biofilm. Lens Frenz Drop B5 eye drops are ineffective against microorganisms and biofilm. Conclusion: Bacteria responsible for keratitis can form biofilms on contact lenses even at low densities. Therefore, it is essential to use eye drops and contact lens solutions regularly and according to the guidelines.