KẾT QUẢ CHUYỂN PHÔI SAU THỰC HIỆN PHƯƠNG PHÁP PHÂN TÍCH DI TRUYỀN KHÔNG XÂM LẤN

Abstract

Objective: To evaluate the results of embryo transfer after performing the non-invasive genetic analysis method (Nonivasive Preimplantation Genetic Testing for Aneuploidy - NiPGT-A). Subjects and methods: A cross-sectional observational study on 44 couples who were indicated for preimplantation genetic testing to screen for aneuploidy (PGT-A) and NiPGT-A who volunteered to participate in the study from 2020-2024 at Tam Anh General Hospital - Hanoi, and were cultured using the single-drop culture process. Results: The average number of aspirated follicle complexes was 11.73 ± 7.13 complexes, the smallest was 2, the largest was 34; MII oocytes accounted for the majority with an average of 8.73 ± 5.76, the smallest was 2, the largest was 28; The average GV oocytes were 0.2 ± 0.15, the smallest was 0, the largest was 1; the average MI oocytes were 0.93 ± 0.93, the smallest was 0, the largest was 4. The average degenerated oocytes were 1.98 ± 2.52, the smallest was 0, the largest was 10. The average number of ICSI oocytes was 8.80 ± 5.73, the smallest was 2, the largest was 28. The fertilization rate was 89% ± 14%. The average N3 cleavage embryo size was 7.84±5.10, the smallest was 2, the largest was 27. The average blastocyst size was 7.82±5.12, the smallest was 2, the largest was 27. The rate of cleavage embryo formation on day 3 was 90.6±13.1%, the rate of blastocyst formation was 90.1±14.5%. A total of 21/44 (47.72%) couples had embryos transferred, of which 25 transfers of only 1 embryo were performed (17 embryos on day 5 (68%), 8 embryos on day 6 (32%)). The pregnancy rate was 90.48%, of which the biochemical pregnancy rate was 12.93%, the clinical pregnancy rate was 76.19%. There were 13 cases of clinical pregnancy with fetal heartbeat, accounting for 61.9%, and the pregnancy progressed to 42.86%. There were 5 cases of retained and miscarried embryos, accounting for 9.52%. Seven cases of live births were recorded, accounting for 33.3%. Conclusion: The pregnancy rate was 90.48%, of which the biochemical pregnancy rate was 12.93% and the clinical pregnancy rate was 76.19%. Thirteen clinical pregnancies had fetal heartbeats, accounting for 61.9% and the pregnancy progressed to 42.86%; five cases of retained and miscarried embryos, accounting for 9.52%. Seven cases of live births were recorded, accounting for 33.3%. Adjusting the single-drop embryo culture process has increased the successful embryo culture rate from the day 3 cleavage stage to the blastocyst stage, ensuring non-invasive pre-implantation genetic testing, contributing to improving the pregnancy rate in general and the clinical pregnancy rate in particular.