NGHIÊN CỨU HOẠT TÍNH CHẾ TIẾT MỘT SỐ CYTOKINE QUAN TRỌNG CỦA TẾ BÀO GIẾT TỰ NHIÊN CÓ NGUỒN GỐC TỪ BỆNH NHÂN UNG THƯ TUYẾN TIỀN LIỆT

Abstract

Objectives: The study aimed to investigate the key cytokines secretion characteristics of natural killer (NK) cells from prostate cancer (PCa) patients using in vitro culture and to assess these characteristics when NK cells are co-cultured with PCa cell lines. Methods: NK cells were isolated from the peripheral blood of PCa patients. These cells were cultured and expanded, and the culture supernatant was collected to evaluate cytokine secretion capabilities. Expanded NK cells were then co-cultured with the PCa cell line (PC3), and the supernatant was harvested at various time points to assess cytokine secretion activity. Results: The concentrations of the cytokines IFN-γ, TNF-α, and GM-CSF exhibited average fold increases of 1.9, 2.7, and 4.5, respectively. The cytokine concentrations in the co-culture supernatant showed significant changes at 24 hours compared to 6 hours and 0 hours. Conclusion: NK cell cytokine levels significantly increased during the expansion process and when NK cells were co-cultured with a prostate cancer cell line