MỐI LIÊN QUAN GIỮA TÌNH TRẠNG ĐỘT BIẾN GEN KRAS, NRAS, BRAF VÀ MỘT SỐ ĐẶC ĐIỂM BỆNH HỌC UNG THƯ BIỂU MÔ ĐẠI TRỰC TRÀNG GIAI ĐOẠN DI CĂN

Abstract

Objective: (1)To determine the rate of KRAS, NRAS and BRAF gene mutations in metastatic colorectal cancer; (2) To explore the possible relationship between their mutation status with some clinicopathological characteristics. Patients and methods: A cross-sectional study included 76 cases of metastasis colorectal cancer were diagnosed and treated at 108 Military Central Hospital. KRAS, NRAS and BRAF mutations were identified by direct DNA sequencing. Results: Among the 76 metastasis colorectal cancer patients, we detected 34 (44,7%) mutations in the KRAS gene, 3 (3,2%) mutations in the NRAS gene, and 7 (9,2%) mutations in the BRAF gene. The KRAS mutation frequency was significantly higher in the patients who had initial CEA level > 5ng/ml (p = 0,022). The BRAF mutation frequency was significantly higher in the patients who had initial CEA level > 20ng/ml (p = 0,007). Other clinicopathological features, such as age, gender, personal status,primary tumor location, histological grade, number of metastatic sites showed no positive relationship with KRAS and BRAF gene mutation status. There was not the positive relationship between gender, primary tumor location, CEA level, histological grade and status of NRAS mutation in our research. Conclusion: The KRAS, NRAS and BRAFmutation rates of metastasis colorectal cancer in 108 Military Central Hospital was 44,7%; 3,2% and 9,2%, respectively. KRAS mutation was associated with CEA level (> 5ng/ml). BRAF mutation was associated with CEA level (> 20ng/ml).