ẢNH HƯỞNG CỦA THỜI GIAN CỐ ĐỊNH MẪU MÔ PHẪU THUẬT ĐẾN BIỂU LỘ CÁC DẤU ẤN ER VÀ HER2 TRONG UNG THƯ BIỂU MÔ TUYẾN VÚ

Abstract

Objective: To compare the influence of fixation time on surgical tissues on routine Hematoxylin & Eosin (H&E) staining results the expression levels of ER and Her2 markers in breast carcinoma. Subjects and methods: A cross-sectional descriptive study was conducted on 30  patients diagnosed with breast carcinoma and operated on at K Hospital. Results: H&E staining obtained 31.1% good samples, 65.5% satisfactory samples and 3.4% unsatisfactory samples. When comparing the similarity of ER markers immunohistochemical (IHC) stains, 2h and 8h fixed samples gave results of 57.7% dissimilarity in intensity and 73.1% dissimilarity in the rate of expression of the marker on the cell nucleus. When comparing the similarity of ER markers between 8h and 16h fixed samples, the results indicated that the intensity and the expression levels were 3.8% and 30,8% dissimilarity, respectively. For Her2 markers, when comparing the similarity between 2h and 8h fixed samples, the results were 26.3% similar and 73.7% dissimilar. When comparing the similarity between 8h and 16h fixed samples, the results were 57.9% similar and 42.1% dissimilar in the intensity of the marker expression on the cell membrane. Conclusion: Fixation time that is inadequate or excessive on the tissue sample, leading to unreliable expression of IHC markers and affecting the conclusion and treatment direction for the patient.