KHẢO SÁT SỰ THAY ĐỔI CÁC THÀNH PHẦN CARBON VÀ OXYGEN TRONG VẬT LIỆU NGÀ RĂNG SAU KHỬ KHOÁNG ĐỂ GHÉP Ổ RĂNG SAU KHI NHỔ

Abstract

Objective: To investigate the changes in the mineral composition of carbon and oxygen in dental enamel after demineralization with 10% ethylenediaminetetraacetic acid (EDTA). Methods: The study was conducted at the Department of Odonto-Stomatology and the Molecular Biomedical Center, University of Medicine and Pharmacy, Ho Chi Minh City, along with the Nanotechnology Department, High-Tech Research and Development Center, Ho Chi Minh City from November 2023 to February 2024. Intact wisdom teeth that met the requirements were collected, divided into root (CR) and whole tooth (NR) groups, and treated according to the protocol. The teeth were then completely ground using a Smart Dentin Grinder machine and demineralized with 10% EDTA according to the specified time points. The collected samples were packaged and analyzed using X-ray energy dispersive spectroscopy to determine the weights of carbon and oxygen in the specimens after demineralizing for 2, 10, 60 min (T60), and 24 h (T1440). Data were processed using SPSS software with appropriate statistical tests. Results: In both groups, the absolute weight of carbon gradually increased over the demineralization time. At T1440, the absolute mass of carbon decreased compared to that at T60, but remained higher than that at T0. The percentage of carbon increased gradually from T0 to T1440 in both CR and NR groups. The change in carbon percentage was statistically significant in both groups. For oxygen, both the absolute weight and percentage by weight gradually decreased over the demineralization time in both CR and NR groups. However, the difference in weight between groups was not statistically significant. Conclusion: The weight of carbon was not affected, whereas that of oxygen decreased over time during demineralization. Changes in the weight of these elements are attributed to the dissolution of hydroxyapatite in the tooth structure.