PHÂN BỐ TYPE HUYẾT THANH, TÌNH HÌNH KHÁNG KHÁNG SINH VÀ ĐẶC ĐIỂM PHÂN TỬ CỦA CÁC CHỦNG STREPTOCOCCUS PNEUMONIAE GÂY BỆNH PHẾ CẦU KHUẨN XÂM LẤN Ở MỘT SỐ BỆNH VIỆN MIỀN BẮC VIỆT NAM

Abstract

Objective: This study aims to  identify serotype distribution of Streptococcus pneumoniae isolated from invasive pneumoniae patients at some hospitals in northern Vietnam from 2014 to 2023, and  to determine molecular characteristics and antibiotic susceptibility profile of S.pneumoniae isolates. Materials and methods: This is a cross-sectional descriptive study, collecting 80 invasive pneumococcal isolates from 2014 to 2023 at some hospitals in northern Vietnam. Antimicrobial susceptibility testing was performed by disk diffusion method and Etest. Whole genome sequencing was carried out on Illumina Miseq. Results: There were 22 serotypes detected in 80 invasive pneumococcal isolates, in which the most ubiquitous serotypes included 9V, 19A, 3, 23F, 19F, 11A, 14, 6B/6E, 15A accounting for 71.3%. Seven out of  these 9 serotypes were covered by vaccin PCV13. We detected 42 different MLSTs with the most prevelent sequence types such as ST10120, ST320, ST180, ST63, ST271, ST166. All S.pneumoniae isolates still maintained sensitive to levofloxacin, but high multiple drug resistance - MDR proportion (90%), and highly non-sensitive to:  erythromycin (92.5%), clindamycin (92.5%), tetracycline (88.8%) and trimethoprim sulfamethoxazole (73.8%). Gene ermB mainly conferred for resistance to erythromycin and clindamycin (91.3%). Conclusions: S.pneumoniae isolates in this study carried many different serotypes and MLSTs, with high rate of MDR strains, and high percentage of resistance to erythromycin, clindamycin and tetracycline