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DEVELOPING A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY TECHNIQUE FOR THE DETERMINATION OF VORICONAZOLE PLASMA CONCENTRATION
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Abstract
Aims. To develop a novel high-performance liquid chromatography (HPLC) technique to determine VRC concentration in human plasma.Methods. The peak of voriconazole (VRC) was detected using an XDB RP C18 (150x4,6mm, 5µm) column, a mobile phase of water: acetonitrile (60:40 v/v), at a flow rate of 1 mL/min. We also report a case of a 66-year-old male to demonstrate the application of this technique in clinical practices at the University Medical Center Ho Chi Minh City. Results. The HPLC method showed good accuracy, specificity, selectivity, and stability. A linear response (R2 = 0,9997) was observed in the range of 0,25–10µg/mL. There was a strong correlation between VRC concentration and clinical efficacy as well as toxicity. Conclusions. The novel technique allowed rapid detection of VRC in human plasma with a lower limit of quantification of 0.25 µg/mL. We have successfully applied this technique in clinical practices to maximize efficacy and minimize toxicity.
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Keywords
HPLC, TDM, voriconazole, invasive fungal diseases
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