Article Sidebar

pdf
Date Published: 26/03/2025
Abstract Views: 105
Views pdf: 48
DOI: https://doi.org/10.51298/vmj.v547i1.12902
Issue
Vol. 547 No. 1 (2025)
Section
Các bài báo
How to Cite
Qúach, T. H. G., Trần, T. H., Vũ, T. P. D., Lê, L. A., Đỗ, T. V. A., & Phạm, T. L. (2025). IDENTIFICATION OF THE FREQUENCY OF B CELLS SUBSETS IN THE PERIPHERAL BLOOD OF PATIENTS WITH PEMPHIGUS VULGARIS. Vietnam Medical Journal, 547(1). https://doi.org/10.51298/vmj.v547i1.12902

IDENTIFICATION OF THE FREQUENCY OF B CELLS SUBSETS IN THE PERIPHERAL BLOOD OF PATIENTS WITH PEMPHIGUS VULGARIS

Thị Hà Giang Qúach, Thị Huyền Trần, Thị Phương Dung Vũ, Lan Anh Lê, Thị Vinh An Đỗ, Thị Lan Phạm

Main Article Content

Abstract

Objective: To identify the number, percentage of B cell subsets in the peripheral blood of patients with pemphigus vulgaris using flow cytometry. Methods: Cross-sectional descriptive study on 82 patients with pemphigus vulgaris. Flow cytometry was used to identify B cell and B cell subsets including B cells, naïve B, transitional B, memory B, switched memory B, unswitched memory B, atypical memory B, plasmblast B, regulatory B, transitional T2 B, double negative. Results:  Age median (Q1-Q3) was 52(43-65), 56(68,29%) female patient and 26(31,71%) male patients. In peripheral blood, number and percentage of naïve B cells was the highest frequency, at 94 cells/μL(48,50%), following memory B 80 cell/ μL(38,55%) including unswitched memory B 31 cell/μL(13,8%), switched memory B 47 cell/μL (22,75%), double negative B 28 cell/μ(13,2%),transitional B 11 cell/μL (5,5%), plasmablast 3 cell/μL (1,5%), Breg  6 cell/μL (2,6%). The number and percentage off atypical memory B was the lowest frequency, 1 cell/μL (0,6%). Conclusion: In the peripheral blood of patients with pemphigus vulgaris, there were several B cells subsets. Changes in the number and proportion of B cells in peripheral blood may contribute to the onset and progression of pemphigus vulgaris

Article Details

Keywords

B cell subsets, flow cytometry, pemphigus vulgaris.

References

Spindler V, Eming R, Schmidt E, et al. Mechanisms Causing Loss of Keratinocyte Cohesion in Pemphigus. J Invest Dermatol. 2018;138(1):32-37. doi:10.1016/j.jid.2017.06.022
2. Lim YL, Bohelay G, Hanakawa S, Musette P, Janela B. Yen Loo Lim, Gerome Bohelay, Sho Hanakawa, Autoimmune Pemphigus: Latest Advances and Emerging Therapies, Front. Mol. Biosci. 8:808536. Front Mol Biosci. 2022;8:26.
3. Yamagami J. B‐cell targeted therapy of pemphigus. J Dermatol. 2023;50(2):124-131. doi:10.1111/1346-8138.16653
4. Liu et al. 2017 - Peripheral CD19hi B cells exhibit activated phenot.pdf.
5. Shimizu T, Takebayashi T, Sato Y, et al. Grading criteria for disease severity by pemphigus disease area index. J Dermatol. 2014;41(11):969-973. doi:10.1111/1346-8138.12649
6. Rosenbach M, Murrell DF, Bystryn JC et al. Reliability and convergent validity of two outcome instruments for pemphigus. J Invest Dermatol 2009; 129: 2404–2410.
7. Morbach H, Eichhorn EM, Liese JG, Girschick HJ. Reference values for B cell subpopulations from infancy to adulthood. Clin Exp Immunol. 2010;162(2): 271-279. doi:10.1111/j.1365-2249. 2010.04206.x
8. Sanz I, Wei C, Jenks SA, et al. Challenges and Opportunities for Consistent Classification of Human B Cell and Plasma Cell Populations. Front Immunol. 2019;10: 2458. doi:10.3389/fimmu. 2019.02458
9. Pollmann R, Walter E, Schmidt T, et al. Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris. Front Immunol. 2019;10: 1375. doi:10.3389/ fimmu.2019.01375
10. Yuan H, Zhou S, Liu Z, et al. Pivotal Role of Lesional and Perilesional T/B Lymphocytes in Pemphigus Pathogenesis. J Invest Dermatol. 2017;137(11): 2362-2370. doi:10.1016/j.jid.2017. 05.032