NGHIÊN CỨU NỒNG ĐỘ CÂN BẰNG KHOÁNG CỦA DUNG DỊCH BẢO QUẢN MẪU MÔ SINH HỌC

Abstract

The process of preserving biological tissues in solutions is always accompanied by metabolic exchanges between the tissues and the preservation solution. Determining the kinetics of calcium and phosphat in the solution, identifying the equilibrium point, and predicting the extent of mineral leaching to propose measures for replenishing mineral concentrations in the preservation solution are crucial. These efforts aim to minimize the demineralization process and maintain the structural integrity of biological tissues. The study titled “Investigation of Mineral Equilibrium Concentrations in Preservation Solutions for Biological Tissue Samples” was conducted with the objectives of: “Developing an experimental model” and “Determining the equilibrium concentrations of calcium and phosphat in the preservation solution for biological tissue samples based on the experimental model.” Subjects and Methods: Eighteen muscle tissue samples (2x2x0.5 cm), 18 spongy bone samples, and 18 compact bone samples (periosteum removed) were immersed in the preservation solution and in solutions supplemented with specific mineral concentrations. The samples were divided into two groups, stored at two different temperatures: 16°C and 37°C. Every 2-4 months, calcium and phosphat concentrations in the preservation solutions were quantified. Results: The initial appropriate mineral concentrations for supplementation were determined to be 30.21 mg/L for calcium and 76.29 mg/L for phosphat. After 27 months of preservation and mineral supplementation, the basic composition and some physicochemical properties of the solution showed negligible changes, confirming its suitability for experimentation. The equilibrium concentrations of calcium and phosphat in the preservation solution were found to be 43±0.5 mg/L and 135±0.5 mg/L, respectively. Conclusion: A long-term experimental model was successfully developed to evaluate the equilibrium concentrations of calcium and phosphat in the preservation solution. At equilibrium mineral concentrations, the solution's composition remained stable, and the leaching of calcium and phosphat from the preserved tissues into the solution was significantly reduced.