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STUDY ON CREATING ACELLULAR BOVINE BONE GRANULES USED FOR DENTAL BONE GRAFTING
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Abstract
Background: In Vietnam, bone grafts used in dental implants are imported from foreign countries. Objective: In this study, we aimed to make decellularized bovine bone granules applied in dental implant. Materials and methods: Bovine femoral cancellous bone was cut and decellularized by the combination of 50% methanol/50% chloroform (MC) for 6 or 24 hours and 0.15% sodium dodecyl sulfate (SDS)/distilled water for 24 hours. Decellularization was tested using Hematoxylin/Eosin and DAPI staining. In vitro cytotoxicity of decellularized bone granules was performed according to ISO 10993-5. Results: The results showed that the optimized decellularization method was the combination of 50% methanol/50% chloroform for 6 hours and 0.15% SDS for 24 hours. The acellular bone granules were not toxic to L-929 cells, according to ISO 10993-5. Conclusion: decellularized bone granules were successfully created, and they have the potential to be used as bone grafts.
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Keywords
cancellous bone, decellularization, methanol/chloroform, SDS, ISO 10993-5
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