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Date Published: 10/03/2025
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DOI: https://doi.org/10.51298/vmj.v547i2.13025
Issue
Vol. 547 No. 2 (2025)
Section
Các bài báo
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Nguyễn, T. M., Hoàng, T. V., & Cấn, H. viện Q. y. (2025). STUDY ON SEQUENCE ANALYSIS TO DETERMINE MUTATIONS AND EXPRESSION LEVELS OF NUCB2 AND SLC2A2 GENES RELATED TO NESFATIN-1 CONCENTRATION IN PATIENTS WITH TYPE 2 DIABETES. Vietnam Medical Journal, 547(2). https://doi.org/10.51298/vmj.v547i2.13025

STUDY ON SEQUENCE ANALYSIS TO DETERMINE MUTATIONS AND EXPRESSION LEVELS OF NUCB2 AND SLC2A2 GENES RELATED TO NESFATIN-1 CONCENTRATION IN PATIENTS WITH TYPE 2 DIABETES

Thị Minh Nguyễn, Trung Vinh Hoàng, Học viện Quân y Cấn

Main Article Content

Abstract

Objective: To determine the gene expression levels and analyze sequencing to identify mutations in certain genes associated with nesfatin-1 levels in patients with type 2 diabetes. Subjects and Methods: The study was conducted with 139 patients with type 2 diabetes in the patient group and 75 subjects in the control group at 198 Hospital; where nesfatin-1 levels were quantified. The methods employed in the study included gene expression analysis using Real-time PCR and Sanger sequencing. Results: The expression levels of the NUCB2 and SLC2a2 genes showed significant changes when comparing the type 2 diabetes patient group with the control group (p<0.05): The NUCB2 gene expression level decreased by about 18-22% compared to the healthy control group. The SLC2A2 gene expression level increased by 121% when compared to the control group. Six mutations were identified in the NUCB2 gene sequence across 6 samples out of the total 10 samples sequenced (rs1941384932 G/A; rs1941406012 G/A; rs945974367 C/A; rs1239838466 G/C; Rs1941390294 G/C; and Rs1476160930 GG/G). Among these, mutations in the intronic region included rs1941384932 G/A, rs1941390294 G/C, and Rs1476160930 GG/G. Three SNP points in the exon region were: rs1941406012 G/A, rs945974367 C/T, and rs1239838466 G/C. In the SLC2a2 gene region, 06 SNPs were recorded in two groups of patients: newly diagnosed and currently treated (rs756099063 T/A; rs2108232309 A/G; rs121909745 C/A; rs201322542 G/A; rs754406000 T/C; and rs370761728 A/T), with 3 points in the exons and 3 points in the introns. Conclusion: This study provides important initial data regarding the presence of SNPs and gene expression levels of NUCB2 and SLC2A2 in patients with type 2 diabetes, related to nesfatin-1 levels in these patients. However, to determine the exact correlation between these two gene regions, further expanded and in-depth studies are needed.

Article Details

Keywords

nesfatin-1; Type 2 diabetes; NUCB2 gene; SLC2A2 gene; newly diagnosed; treated.

References

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