XÂY DỰNG QUY TRÌNH SẢN XUẤT MẪU NƯỚC TIỂU GIẢ ĐỊNH VỚI CÁC THÔNG SỐ: PROTEIN, HỒNG CẦU, BẠCH CẦU, NITRIT ỨNG DỤNG TRONG NGOẠI KIỂM TỔNG PHÂN TÍCH NƯỚC TIỂU

Abstract

Objective: To develop the procedure of abnormal simulated urine production for urinalysis EQA scheme and evaluate the homogeneity as well as stability of producing urine samples in storage conditions.  Methods: Two batches of simulated urine have produced, one set was adjusted to be positive with protein (PRO) and red blood cell (BLO), the other was positive with leukocyte (LEU) and nitrite (NIT) parameters. The producing samples were evaluated the homogeneity and long-term stability in different storage conditions (2-8°C and 25-30°C). Results: Procedure of abnormal simulated urine production has successfully developed. Two sets of simulated urine have been produced and evaluated homogeneity and stability. At 2-8°C, the samples was stable in 12 weeks. However, at 25-30°C, the PRO, BLO, LEU parameters reduced and NIT turned to be negative in 1 week. Conclusion: Procedure of simulated urine production has been established and utilized to produce mass samples. The producing samples reached the criteria in term of homogeneity and stability in 3 months at 2-8°C. These samples are suitale to apply in urianalysis EQA program.