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Date Published: 02/04/2025
Abstract Views: 82
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DOI: https://doi.org/10.51298/vmj.v547i3.13222
Issue
Vol. 547 No. 3 (2025)
Section
Các bài báo
How to Cite
Nguyễn Hữu Ngọc, T., Nguyễn Hưng, T., & Nguyễn Thanh, N. (2025). ESTABLISHING A MODEL OF EXPERIMENTAL STEATOSIS IN VITRO IN HEPG2 CELL BY VALPROIC ACID. Vietnam Medical Journal, 547(3). https://doi.org/10.51298/vmj.v547i3.13222

ESTABLISHING A MODEL OF EXPERIMENTAL STEATOSIS IN VITRO IN HEPG2 CELL BY VALPROIC ACID

Tuấn Nguyễn Hữu Ngọc, Thịnh Nguyễn Hưng, Ngân Nguyễn Thanh

Main Article Content

Abstract

Introduction: The in vitro model is an effective method for studying liver pathophysiology and is also applied as a tool for evaluating drug use on the liver. This research aims to establish an in vitro hepatic steatosis cell model based on the HepG2 cell line stimulated with valproic acid. This model can be applied to studies related to non-alcoholic fatty liver disease. Methods: This research involved an experimental investigation of the HepG2 cell line (obtained from the American Type Culture Collection, catalog number HB-8065TM). The study focused on optimizing cell seeding density for stimulation, as well as determining the ideal concentration and harvest time to achieve efficient lipid accumulation while maintaining cell viability. Results: The in vitro model of steatosis induction on the HepG2 cell line in DMEM-no glucose with 10% FBS and 50μg/mL Gentamicin was established with an initial cell density of 4.104 cells/mL, stimulated by 5mM valproic acid for 48 hours. Conclusion: The study has successfully established a highly efficient and reproducible in vitro model of steatosis using HepG2 cells.

Article Details

Keywords

in vitro, steatosis, HepG2, valproic acid

References

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