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CARBAPENEMASE GENE AND MULTI-DRUG RESISTANCE OF ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALIZED COMMUNITY-ACQUIRED PNEUMONIA PATIENTS
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Abstract
Background: The treatment of community-acquired pneumonia caused by Acinetobacter baumannii is difficult due to the high rate of multi-antibiotic resistance, especially strains carrying genes associated with carbapenenase production. Aims: The aims is determine the carbapenemase genes and relationships between genes in meropenem resistance. Methods: This study was a cross-sectional descriptive design in prospect conducted on A. baumannii strains isolated from sputum of hospitalized community-acquired pneumonia patients. The research method is traditional culturing to determine antibiotic MICs and multiplex real-time PCR technique to identify genes encoding carbapenemase. Results: The proportion of A. baumannii carrying carbapnenemase genes was 91.4%, of which the OXA-51 gene accounted for the highest proportion at 85.7% and OXA-58 gene got the lowest proportion at 17.1%. A. baumannii strains carrying ³ 2 genes reached at 68.8% and had a statistically significant higher rate of antibiotic resistance than strains carrying 1 gene (p < 0.05). The proportion of 12 A. baumannii strains (37.5%) carrying the couple genes OXA-51/23 were 100% resistant to meropenem. Most A. baumannii strains were > 65% resistant to carbapenem antibiotics, 3rd generation cephalosporins, aminoglycosides and colistin. Conclusions: The rate of A. baumannii carrying carbapenemase genes is 91.4%, of which the OXA-51 gene reach at 85.7% and strains carrying ³ 2 genes have a statistically significant higher rate of antibiotic resistance than strains carrying 1 gene (p < 0.05).
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Keywords
carbapenemase - producing Acinetobacter baumannii, gene co - operation, antibiotic resistance
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