KHẢO SÁT KHẢ NĂNG KHÁNG KHUẨN CỦA SỢI HUYẾT GIÀU TIỂU CẦU CẢI TIẾN ĐỐI VỚI STAPHYLOCOCCUS AUREUS

Abstract

Objective: To examine the antibacterial capacity of advanced platelet-rich fibrin plus (A-PRF+) against methicillin-suspectible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) using agar diffusion experiment. Method: This in vitro study examined 15 A-PRF+ samples of male volunteers who satisfied inclusion and exclusion criteria. The Culti-loops S. aureus code ATCC33591 (MRSA) and ATCC6538 (MSSA) were used for experiment. Bacteria was activated with guidance from the manufacture. Then, bacteria inocula were prepared at concentration of 0.5 McFarland.  The A-PRF+ mass was produced according to the centrifugation protocol of Choukroun and Gnanaati (2018). The experiment was conducted on Luria-Bertani agar disk. The positive control was amoxicillin 0.01 mg/mL, wherares the negative control was phisiology saline. The inhibition zone was recorded and measured at 24 hours after incubating in approriate condition. Results: The dimension of inhibition zone of A-PRF+ against MSSA was significantly smaller than that of amoxicillin (19.38 ± 23.62 versus 177.34 ± 23.50; p < 0.001). Similarly, the dimension of inhibition zone of A-PRF+ against MRSA was significantly smaller than that of amoxicillin (15.32 ± 17.70 versus 186.88 ± 65.90; p < 0.001). No inhibition zone was found in negative control group. Conclusion: This in vitro study result suggest that A-PRF+ has weak antibacterial capacity against MSSA and MRSA.