TÌNH TRẠNG NHIỄM HUMAN PAPILLOMAVIRUS TRÊN MẪU MÔ CỦA BỆNH NHÂN UNG THƯ PHỔI

Abstract

Objective: Determine HPV infection on UTP tissues using the Sanger sequencing method and initially evaluate the relationship between HPV infection and the survival rate of lung cancer patients. Subjects and Methods: Cross-sectional, retrospective descriptive study on 100 lung biopsy specimens of patients with primary non-small cell lung cancer from 2018 - 2019, stored at Bach Mai Hospital, conducted at the Gene-Protein Research Center (Hanoi Medical University). Results: Men accounted for 81%, and women accounted for 19%. The majority were ≥ 60 years old, average age 60.57 ± 8.36. The rate of EGFR gene mutations is 33%. The majority were stage III-IV (93%), 77% were smokers, and 98% had adenocarcinoma. The extracted DNA has a purity ensuring PCR and gene sequencing. 9 positive samples were detected by amplification with a specific pair of primers in PCR reaction and 2% agarose gel electrophoresis. After gene sequencing, 6 samples have consistent results with the L1 gene, with HPV type 44. HPV infection was associated with lower median survival time and survival rate, but not statistically significant. Conclusion: The study determined that the prevalence of HPV infection in 100 non-small cell lung cancer samples was 6%. HPV infection was associated with lower median survival time and survival rate, but not statistically significant.