PHÂN LẬP VÀ NUÔI CẤY NGUYÊN BÀO SỢI TẠI KHOA RĂNG HÀM MẶT, ĐẠI HỌC Y DƯỢC THÀNH PHỐ HỒ CHÍ MINH

Abstract

Introduction: The palatal mucosa constitutes a prime source for the isolation of fibroblast cells, facilitating cutting-edge research and therapeutic interventions. The procurement of cells from this region presents numerous advantages, including streamlined sampling protocols, expedited wound healing and minimal cicatrization. Previous studies conducted in Vietnam have focused on harvesting cells from dental pulp, gingival tissue, dental papilla and periodontal ligaments. The cultivation of fibroblasts from palatal mucosa promises to provide an additional reservoir of mesenchymal cells, thereby augmenting both foundational research and clinical applications. Objectives: Culture and isolation of primary human oral fibroblast from palatal mucosa in vitro. Materials and methods: Autologous soft tissue grafting specimens were harvested from patients undergoing surgical procedures, utilizing palatal mucosa as the donor site. Subsequent cell isolation and cultivation adhered strictly to protocols established by the Oral and Maxillofacial Biology Laboratory at the University of Medicine and Pharmacy at Ho Chi Minh City. Results: This tissue culture methodology facilitated the isolation of fibroblast cells, reaching culturing density after 14 days of in vitro cultivation. Conclusion: Primary fibroblast cells from human gingival palatal tissue were effectively isolated and cultivated in vitro.