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EVALUATION OF THE ASSOCIATION BETWEEN F. NUCLEATUM, B. FRAGILIS, E. COLI, AND A. MUCINIPHILA IN TUMOR TISSUE WITH KRAS, BRAF, AND HSP110 GENE MUTATIONS IN PATIENTS WITH COLORECTAL CANCER
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Abstract
Objective: To evaluate the association between the bacteria Fusobacterium sp., E. coli, Bacteroides fragilis, Akkermansia muciniphila in tumor tissue and gene mutations in KRAS, BRAF, and HSP110 in patients with colorectal cancer (CRC). Methods: A cross-sectional descriptive study was conducted on 139 CRC patients. The presence of KRAS, BRAF, and HSP110 mutations was identified using Real-time PCR, along with detection of the bacteria Fusobacterium sp., E. coli, Bacteroides fragilis, and Akkermansia muciniphila in tumor tissue. Results: Analysis showed no significant difference in the bacterial load of B. fragilis between CRC tumor tissues with and without BRAF and HSP110 mutations. However, the B. fragilis load in tumors with KRAS mutation was significantly higher compared to those without the mutation (p = 0.037). While co-infection of B. fragilis + E. coli showed a statistically significant association with KRAS mutation (p = 0.012). Conclusion: Our preliminary findings indicate that B. fragilis and co-infection with B. fragilis+E.coli are associated with colorectal cancer.
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Keywords
Colorectal cancer, Fusobacterium sp., E. coli, Bacteroides fragilis, Akkermansia muciniphila
References
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