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EVALUATION OF RT-LAMP AND RT-PCR EFFICIENCY FOR DETECTION AND SEROTYPING OF DENGUE VIRUS
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Abstract
Objectives: Evaluation of the efficiency of RT-LAMP in comparison with RT-PCR in detection and serotyping of dengue virus in clinical samples. Materials and methods: 95 blood sera collected from 04/2023 to 10/2023 were tested for the presence of dengue virus RNA and analyzed the serotype by both RT-PCR and RT-LAMP methods. Results: The result indicated high concordance (κ= 0,94 ± 0.04) between the two methods. The sensitivity, specificity, positive predictive value, negative predictive value of RT-LAMP were 97,3%, 100%, 100% và 91,3%, respectively. Conclusion: RT-LAMP is an effective method for detection and serotyping dengue virus in clinical samples. RT-LAMP is simple to perform, allow detection and serotyping of dengue virus in a single reaction with minimun requirement of equipment, thus, capable to replace RT-PCR for rapid dengue fever diagnosis on-site or at regions with limited infrastructure.
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Keywords
dengue virus, dengue fever, RT-PCR, RT-LAMP
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