ĐÁNH GIÁ MẪU NỘI KIỂM TRA CHẤT LƯỢNG XÉT NGHIỆM ĐỊNH NHÓM MÁU HỆ ABO VÀ RHESUS D TRÊN BA PHƯƠNG PHÁP: PHIẾN ĐÁ, ỐNG NGHIỆM VÀ GELCARD

Abstract

Objective: This study aimed to evaluate internal control samples for ABO and Rhesus D blood group determination across three methods: slide, test tube and gelcard. Methods: The internal control samples consisted of 8 lots, each containing 9 samples, including red blood cells A Rhesus D positive, B Rhesus D positive, AB Rhesus D positive, O Rhesus D negative, A plasma, B plasma, AB plasma and O plasma. These lots were evaluated using three blood grouping methods: slide, test tube and gelcard. From each lot, three samples were randomly selected and tested across all three methods, followed by comparision of the concordance rate among the methods. Results: All three testing methods: slide, test tube and gelcard accurately determined the blood groups of internal control sample sets for the ABO and Rhesus: A Rhesus D positive red blood cells, B Rhesus D positive red blood cells, AB Rhesus D positive red blood cells, O Rhesus D negative red blood cells, A plasma, B plasma, AB plasma and O plasma. The concordance rate among the eight sample lots across the three methods reached 100% for all three methods with no discrepancies observed. Conclusions: This study demonstrated a 100% concordance rate in blood group determination of eight internal control sample lots across three testing methods: slide, test tube and gelcard. The internal control samples were found to be compatible with multiple blood grouping methods commonly used in laboratories and a suitable for implementation in internal quality control programs for blood group determination.