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DIAGNOSTIC VALUE AND SEROPREVALENCE OF TOXOCARIASIS USING ELISA AT THONG NHAT DONG NAI HOSPITAL
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Abstract
Objectives: This study aimed to assess the diagnostic performance of the ELISA test in detecting Toxocara spp. infection among patients at Thong Nhat Dong Nai Hospital, as well as to determine the seroprevalence and associated risk factors. Materials and Methods: A cross-sectional study was conducted on 300 patients with suspected Toxocara spp. infection. Serum samples were analyzed using an indirect ELISA system (Multiskan FC). Diagnostic parameters—including sensitivity, specificity, and positive category agreement (PCA)—were evaluated using SPSS version 22.0. Results: The seroprevalence of Toxocara spp. infection was 45.3%. The ELISA test demonstrated high diagnostic performance, with a sensitivity of 92.5%, specificity of 89.8%, and PCA of 95.6%. Significant risk factors associated with seropositivity included male gender (p < 0.05), occupation as a farmer (p < 0.05), and residence in rural areas (p < 0.01). Conclusion: The ELISA test utilizing the Multiskan FC system exhibited high sensitivity and specificity, supporting its utility as a reliable tool for the serological diagnosis of Toxocariasis. The elevated infection rates in high-risk groups underscore the need for targeted preventive strategies.
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Keywords
Toxocara spp., ELISA, seroprevalence, immunoassay
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