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ASSOCIATION OF A20, CYLD AND CEZANNE GENE POLYMORPHISMS WITH INTERLEUKIN – 6, TNF- ALPHA, AND TGF- BETA LEVELS IN PATIENTS WITH LYMPHOCYTIC LEUKEMIA
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Abstract
Objective: Assessing the association between A20, CYLD, and CEZANNE gene polymorphisms and plasma levels of Interleukin-6 (IL-6), TNF-alpha (TNF-α), and TGF-beta (TGF-β) in patients with lymphoid leukemia. Methods: A cross-sectional descriptive study was conducted on 57 patients with acute lymphoblastic leukemia (ALL) and 50 patients with chronic lymphocytic leukemia (CLL) at the National Institute of Hematology and Blood Transfusion. IL-6, TNF-α, and TGF-β levels were quantified using the Sandwich ELISA method. Gene expression was measured by RT-PCR, and gene polymorphisms were identified using Sanger sequencing. Results: The median of IL-6 concentration in the ALL group was higher than that in the CLL group (9.77 pg/mL vs. 5.63 pg/mL), whereas the median of TGF-β concentration in the ALL group was lower than that in the CLL group (534.53 pg/mL vs. 221.17 pg/mL), with p < 0.001. Five A20 polymorphisms, five CYLD polymorphisms, and four CEZANNE polymorphisms were identified. Among them, A20 polymorphisms Chr6137878507 (rs374987145) and Chr6137878514 (rs2114496684) were associated with increased TNF-β levels (p < 0.05), and CYLD polymorphism Chr1650791688 was associated with increased TNF-α levels (p = 0.037) in ALL patients. In CLL patients, CEZANNE polymorphism Chr1149947519 was associated with increased TNF-α (p = 0.036), and Chr1149947613 was associated with increased TNF-β (p = 0.047). Conclusion: Several polymorphisms in A20, CYLD, and CEZANNE genes are associated with altered inflammatory cytokine levels, potentially contributing to changes in the pathogenesis and clinical manifestation of lymphoid leukemia.
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Keywords
Inflammatory cytokines, lymphoid leukemia, deubiquitinase gene polymorphisms
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