NGHIÊN CỨU NỒNG ĐỘ ACID Β-HYDROXYBUTYRIC BẰNG PHƯƠNG PHÁP ĐỘNG HỌC ENZYME TRÊN BỆNH NHÂN ĐÁI THÁO ĐƯỜNG TẠI BỆNH VIỆN CHỢ RẪY

Abstract

Objective: This study aimed to determine the serum β-hydroxybutyric acid (BHB) concentration using the enzymatic kinetic method and to investigate its correlation with HbA1c and ketone levels in diabetic patients with ketoacidosis. Subjects and Methods: A cross-sectional descriptive study was conducted on 103 diabetic patients (case group) and 32 healthy individuals (control group) at Cho Ray Hospital from 07/2023 to 07/2024. BHB concentration was measured by the enzymatic kinetic method, HbA1c by high-performance liquid chromatography, and ketones by a semi-quantitative method. Results: The mean BHB concentration in the case group was 4.42 ± 3.51 mmol/L, which was significantly higher than the control group (0.04 ± 0.03 mmol/L) (p < 0.001). BHB showed a strong positive correlation with blood glucose (r = 0.71; p < 0.001) and semi-quantitative blood ketones (r = 0.71; p < 0.001). No significant correlation was found between BHB and HbA1c (r = 0.111; p = 0.264). During treatment monitoring, the mean BHB concentration decreased significantly from 4.43 mmol/L (first test) to 1.91 mmol/L (second test) and 1.0 mmol/L (third test). Conclusion: BHB concentration measured by the enzymatic kinetic method correlates strongly with blood glucose, better reflects the severity of metabolic disturbance, and provides better therapeutic monitoring value than the semi-quantitative ketone method.