KẾT QUẢ REALTIME-PCR ĐA MỒI XÁC ĐỊNH VI KHUẨN Ở NGƯỜI BỆNH VIÊM PHỔI MẮC PHẢI CỘNG ĐỒNG NHẬP VIỆN TẠI BỆNH VIỆN QUÂN Y 103

Abstract

Objective: To evaluate bacterial etiology using multiplex Realtime-PCR in hospitalized patients with community-acquired pneumonia (CAP) at Military Hospital 103. Subjects and methods: This is a descriptive, prospective study of 34 CAP patients treated at the Medical Respiratory Center and the Internal Intensive Care Unit, Military Hospital 103 from July 2024 to October 2025. Patients underwent clinical examination, blood test, chest Xray and semi-quantitative bacterial culture and multiplex Realtime-PCR sputum testing. Results: The average age was 64.06 ± 16.94 years old, with a male/female ratio of 2.4/1. Symptoms included fever (82.35%), cough (91.17%), and dyspnea (70.58%), lung consolidation (100%), consolidation area ≥ 2 lung lobes (52.94%), high increased leukocytes and CRP. The detection rate of a single bacterial agent using the multiplex Realtime-PCR assay was 52.94%. The highest detection proportion was for S. pneumoniae (52.94%). The concordance between culture results and multiplex Realtime-PCR was 100%. Conclusions: Hospitalized CAP patients were typically male, elderly, having multiple comorbidities, and presenting with various symptoms. Multiplex Realtime-PCR detected a wider range of bacteria than culture. S. pneumoniae was the most common (52.94%). Bacterial culture results were consistent with multiplex Realtime-PCR.