HIỆU QUẢ CỦA PHƯƠNG PHÁP HOẠT HÓA NOÃN NHÂN TẠO CHỦ ĐỘNG VÀ BỊ ĐỘNG

Abstract

Background and aims: In spite of the success of intracytoplasmic sperm injection (ICSI), the total fertilization failure still occurs in 1–5% of all ICSI cycles. The combination between ICSI and the assisted oocyte activation (ICSI-AOA) can restore fertilization. However, the indications of AOA still vary between researches. The goal of this study is investigate the effect of active and rescue AOA applications. Methods: Retrospective research conducted on 427 oocytes of 57 couples in ART center in Vinmec Times City Hospital. Active AOA is performed on patients having a history of low fertilization rates and low quality sperm samples. Rescue AOA is carried out in cases of fertilization failure on ICSI day (D1) and in vitro matured oocytes. Results: In the active AOA group, the rate of fertilization increases significantly and reaches 79% in cases with low fertilization rate (<50%) in history (p<0,05). In rescue AOA group, in cases of currently low fertilization rate (<50% in D0), the fertilization rate rises to 76,5% on the day after ICSI day (DI) (p<0,05); regarding to IVM group, the rate of fertilizations with AOA and without AOA are 67,61% và 50% respectively (p<0,05). Conclusions: AOA can be carried out not only actively on patients with a history of low fertilization rates and in cases of low quality sperm samples, but also passively in cases of fertilization failure on D1 and in vitro matured oocytes. The bio-safety in long term of AOA has been researching, the clinical application of AOA should not be indicated regularly for all infertility patients.