Article Sidebar

PDF
Issue: Vol. 566 No. special (2026): Hệ thống BVĐK Tâm Anh
Section: Các bài báo
DOI: 10.51298/vmj.v566ispecial.19074
Date Published: 06/10/2026
Views 0
Downloads 0
How to Cite
Trần, T. D., Lê, H. H., Nguyễn, X. H., Nguyễn, T. B., Nguyễn, T. L., Trịnh, T. T., Trịnh, T. V., Phạm, T. T., Nguyễn, T. H., Nguyễn, T. H., Nguyễn, T. H. N., & Thẩm, T. T. N. (2026). COMPARISON OF DIFFERENT PROCESSING METHODS AND CRYOPRESERVATION SOLUTION FOR HUMAN UMBILICAL CORD TISSUE AS A SOURCE OF MESENCHYMAL STEM CELLS. Vietnam Medical Journal, 566(special). https://doi.org/10.51298/vmj.v566ispecial.19074

COMPARISON OF DIFFERENT PROCESSING METHODS AND CRYOPRESERVATION SOLUTION FOR HUMAN UMBILICAL CORD TISSUE AS A SOURCE OF MESENCHYMAL STEM CELLS

Thùy Dương Trần1, Hoàng Hà Lê1, Xuân Hưởng Nguyễn1, Thị Bích Nguyễn1, Tâm Long Nguyễn1, Thanh Thảo Trịnh1, Tuấn Việt Trịnh1, Thị Thu Phạm1, Thị Huyền Nguyễn1, Trung Hiệp Nguyễn1, Thị Hằng Nga Nguyễn1, Thị Thu Nga Thẩm1,
1 Bệnh viện Đa khoa Tâm Anh Hà Nội

Main Article Content

Abstract

Cryopreservation of intact umbilical cord tissue is considered an optimal strategy for long-term preservation of a promising source of mesenchymal stem cells (MSCs) for regenerative medicine. This study aimed to evaluate and compare the effects of two tissue-processing sizes (1×1×1 mm and 5×2×2 mm) combined with two types of cryoprotective media (commercial medium and umbilical cord blood plasma, both supplemented with 10% DMSO) on the efficiency of umbilical cord tissue cryopreservation. Experimental results demonstrated that MSCs were successfully isolated from all cryopreserved tissue samples thawed at 1, 3, 6 months, while maintaining their biological properties (morphology, growth kinetics, immunophenotype, and differentiation potential) comparable to the fresh control group (p > 0,05). This study demonstrates the safety and efficacy of the tested cryopreservation methods for umbilical cord tissue, establishing a solid foundation for long-term storage of umbilical cord tissue in tissue banks.

Article Details

References

1. Arutyunyan, Irina, Fatkhudinov, Timur, and Sukhikh, Gennady. “Umbilical cord tissue cryopreservation: a short review”, Stem Cell Research & Therapy. 2018, 9(1), p. 236.
2. Choudhery, M. S., Badowski, M., Muise, A., and Harris, D. T.. “Utility of cryopreserved umbilical cord tissue for regenerative medicine”, Curr Stem Cell Res Ther. 2013, 8(5), pp. 370-80.
3. Dulugiac, M., Moldovan, L., and Zarnescu, O. “Comparative studies of mesenchymal stem cells derived from different cord tissue compartments - The influence of cryopreservation and growth media”, Placenta. 2015, 36(10), pp. 1192-203.
4. Margiana, Ria, Markov, Alexander, Zekiy, Angelina O., Hamza, Mohammed Ubaid, Al-Dabbagh, Khalid A., Al-Zubaidi, Sura Hasan, Hameed, Noora M., Ahmad, Irshad, Sivaraman, R., Kzar, Hamzah H., Al-Gazally, Moaed E., Mustafa, Yasser Fakri, and Siahmansouri, Homayoon. “Clinical application of mesenchymal stem cell in regenerative medicine: a narrative review”, Stem Cell Research & Therapy. (2022), 13(1), p. 366.
5. Mebarki, Miryam, Abadie, Camille, Larghero, Jérôme, and Cras, Audrey. “Human umbilical cord-derived mesenchymal stem/stromal cells: a promising candidate for the development of advanced therapy medicinal products”, Stem Cell Research & Therapy. 2021, 12(1), p. 152.
6. Raffo, D., Maglioco, A., and Fernandez Sasso, D. “A protocol for umbilical cord tissue cryopreservation as a source of mesenchymal stem cells”, Mol Biol Rep. 2021, 48(2), pp. 1559-1565.
7. Romanov, Yu A., Balashova, E. E., Volgina, N. E., Kabaeva, N. V., Dugina, T. N., and Sukhikh, G. T. “Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue”, Bulletin of Experimental Biology and Medicine. 2016, 160(4), pp. 530-534.
8. Roy, S., Arora, S., Kumari, P., and Ta, M. “A simple and serum-free protocol for cryopreservation of human umbilical cord as source of Wharton’s jelly mesenchymal stem cells”, Cryobiology. 2014, 68(3), pp. 467-72.
9. Shivakumar, S. B., Bharti, D., Subbarao, R. B., Jang, S. J., Park, J. S., Ullah, I., Park, J. K., Byun, J. H., Park, B. W., and Rho, G. J. “DMSO- and Serum-Free Cryopreservation of Wharton’s Jelly Tissue Isolated From Human Umbilical Cord”, J Cell Biochem. 2016, 117(10), pp. 2397-412.
10. Skiles, M. L., Brown, K. S., Tatz, W., Swingle, K., and Brown, H. L.. “Quantitative analysis of composite umbilical cord tissue health using a standardized explant approach and an assay of metabolic activity”, Cytotherapy. 2018, 20(4), pp. 564-575.