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Issue: Vol. 565 No. 1 (2026)
Section: Các bài báo
DOI: 10.51298/vmj.v565i1.19239
Date Published: 03/11/2026
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How to Cite
Võ T. N., Trương, Q. H. M., Sử T. H., Đỗ L. C., & Phan, H. N. D. (2026). INVESTIGATION OF CAUSATIVE PATHOGENS IN PERSISTENT PNEUMONIA IN CHILDREN AT CHILDREN’S HOSPITAL OF HO CHI MINH CITY USING BRONCHOALVEOLAR LAVAGE RT-PCR TECHNIQUE. Vietnam Medical Journal, 565(1). https://doi.org/10.51298/vmj.v565i1.19239

INVESTIGATION OF CAUSATIVE PATHOGENS IN PERSISTENT PNEUMONIA IN CHILDREN AT CHILDREN’S HOSPITAL OF HO CHI MINH CITY USING BRONCHOALVEOLAR LAVAGE RT-PCR TECHNIQUE

Thành Nhân Võ1, , Quốc Hoàng Minh Trương2, Thị Hằng Sử1, Linh Chi Đỗ1, Hữu Nguyệt Diễm Phan3
1 Bệnh viện Nhi Đồng Thành Phố
2 Đại học Nguyễn Tất Thành
3 Đại học Y Dược thành phố Hồ Chí Minh

Main Article Content

Abstract

Objectives: To determine the detection rate and characteristics of microorganisms using Real-time PCR and conventional culture from bronchoalveolar lavage (BAL) fluid.


Methods: A case-series observational study was conducted from October 2022 to July 2023 at Children’s Hospital of Ho Chi Minh City. A total of 30 BAL samples for PCR testing were collected from 29 pediatric patients.


Results: The positive culture rate of BAL samples was 50%, and most isolates were hospital-acquired multidrug-resistant pathogens, including Staphylococcus aureus (13.3%), Acinetobacter baumannii and Pseudomonas aeruginosa (6.7%), Klebsiella pneumoniae, Streptococcus pneumoniae, Staphylococcus epidermidis, Haemophilus influenzae, Escherichia coli, and Citrobacter freundii (3.3%).
The pathogen detection rate of PCR was 86.7%, significantly higher than conventional culture methods (50%) (p = 0.007). Most bacteria identified by PCR were hospital-acquired pneumonia pathogens, including Stenotrophomonas maltophilia (47%), Escherichia coli (43%), Streptococcus pneumoniae (30%), Fusobacterium nucleatum (27%), Haemophilus influenzae and Pseudomonas aeruginosa (23%), Staphylococcus aureus and Staphylococcus epidermidis (20%), Klebsiella pneumoniae, Acinetobacter baumannii, and coagulase-negative Staphylococcus (17%).


Conclusion: Pathogen identification using BAL Real-time PCR should be integrated into clinical practice to guide treatment decisions for pediatric patients with persistent pneumonia.

Keywords

Persistent pneumonia, Children, RT-PCR technique, Bronchoalveolar lavage

Article Details

References

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