EVALUATING THE EFFECT OF STAINING THE INTERNAL LIMITING MEMBRANE BY THE MIXTURE OF TRYPAN BLUE 0.08% AND GLUCOSE 10% AT A RATIO 1:1

Trương Văn Bạn1,, Nguyễn Thị Nhất Châu1
1 Central Eye Hospital

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Abstract

Objectives:  To report on the use of the mixture of trypan blue 0.08% and glucose 10% for the staining the internal limiting membrane during vitrectomy. Subjectives and Medthod: 27 consecutive patients with macular hole of stage 2, 3, 4 with different etiologies were recruited for vitrectomy and ILM peel in Retinal Department of VNIO during the period of 1/2015 to 9/2015. ILM peeling was done with the mixture of trypan blue 0.08% and glucose 10% 1:1 assisted. The results was evaluated based on the ability of internal limiting membrane staining and the possibility of totally peeling membrane during the surgery. Postoperatively, the results was evaluated based on anatomical and functional outcomes. Results: The mixture of trypan blue 0.08% and glucose 10% 1:1 provided mediocre but good enough staining of internal limiting membrane for macular hole surgery: 88.9% overage ILM staining, 99.3% of eyes got total ILM peeling during the surgery. 3 months post-op, 85.2% of eyes got total macular hole close, 14,8% of eyes got partial macular hole close. All of the eyes got improvement in visual acuity, 66.7% of eyes got visual acuity improvement of more than 2 lines of Snellen chart. Conclusion: The mixture of trypan blue 0.08% and glucose 10% 1:1 provided mediocre but good enough staining of internal limiting membrane for macular hole surgery.

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References

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