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Date Published: 15/04/2022
Abstract Views: 214
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DOI: https://doi.org/10.51298/vmj.v511i1.2087
Issue
Vol. 511 No. 1 (2022)
Section
Các bài báo
How to Cite
Văn An, N. ., Thu Hồng, L. ., Hùng Cường, N. ., Xuân Quảng, H. ., & Thái Sơn, N. . (2022). COMPARISON OF ABILITY TO DETECT SARS-COV-2 IN 20-POOLED AND 10-POOLED NASOPHARYNGEAL SWABS USING THE REAL-TIME RT PCR METHOD. Vietnam Medical Journal, 511(1). https://doi.org/10.51298/vmj.v511i1.2087

COMPARISON OF ABILITY TO DETECT SARS-COV-2 IN 20-POOLED AND 10-POOLED NASOPHARYNGEAL SWABS USING THE REAL-TIME RT PCR METHOD

Nguyễn Văn An1,, Lê Thu Hồng1, Nguyễn Hùng Cường1, Hoàng Xuân Quảng1, Nguyễn Thái Sơn1
1 Military Hospital 103

Main Article Content

Abstract

Objective: Comparison of ability to detect SARS-CoV-2 in 20-pooled and 10-pooled Nasopharyngeal swabs using the real-time RT PCR method. Methods: This was a descriptive and experimental study. The 10-pooled and 20-pooled samples were created from nasopharyngeal swabs confirmed as positive and negative with SARS-CoV-2. The pooled samples were tested using the real-time RT PCR. Positive agreement and negative agreement of the test on 10-pooled and 20-pooled samples were compared. Results: The percentage of positive agreement of the real-time RT PCR to detect SARS-CoV-2 in 20-pooled with 10-pooled samples was 100%. Conclusion: Our study showed that the ability to detect a SARS-CoV-2 positive sample of the real-time RT PCR method in 20-pooled samples that were produced by mixing the solution of 2 of 10-pooled swab samples was equivalent to the ability to detect a SARS-CoV-2 positive sample in the 10-pooled sample.

Article Details

Keywords

SARS-CoV-2, Real-time RT PCR, COVID-19

References

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